1984年から2003年3月の発表論文リストです。
- Ohtsuka, E., Tozuka, Z., Iwai, S., and Ikehara, M. (1982) A new condensing reagent, 1-(2,4,6-triisopropylbenzenesulfonyl)-5-(pyridin-2-yl)tetrazolide and its use in the synthesis of lambda cro binding heptadecanucleotide on a polymer support. Nucleic Acids Res. 10, 6235-6241
- Ohtsuka, E., Taniyama, Y., Iwai, S., Yoshida, T., and Ikehara, M. (1984) Deoxyribonucleic acids and related compounds. VIII. Solid-phase synthesis of deoxyribooligonucleotides with 3′-modification by elongation in the 3′-direction. Chem. Pharm. Bull. 32, 85-93
- Ikehara, M., Ohtsuka, E., Tokunaga, T., Taniyama, Y., Iwai, S., Kitano, K., Miyamoto, S., Ohgi, T., Sakuragawa, Y., Fujiyama, K., Ikari, T., Kobayashi, M., Miyake, T., Shibahara, S., Ono, A., Ueda, T., Tanaka, T., Baba, H., Miki, T., Sakurai, A., Oishi, T., Chisaka, O., and Matsubara, K. (1984) Synthesis of a gene for human growth hormone and its expression in Escherichia coli. Proc. Natl. Acad. Sci. USA 81, 5956-5960
- Tokunaga, T., Iwai, S., Gomi, H., Kodama, K., Ohtsuka, E., Ikehara, M., Chisaka, O., and Matsubara, K. (1985) Expression of a synthetic human growth hormone gene in yeast. Gene 39, 117-120
- Ohtsuka, E., Iwai, S., Tokunaga, T., and Ikehara, M. (1985) Deoxyribonucleic acids (DNA) and related compounds. XIII. Synthesis of DNA duplexes containing a ribosome binding site. Chem. Pharm. Bull. 33, 3153-3159
- Iwai, S., Asaka, M., Inoue, H., and Ohtsuka, E. (1985) A new solid-phase synthesis of ribooligonucleotides using the 3′-phosphoro-p-anisidate protecting group for stepwise synthesis in the 3′-direction. Chem. Pharm. Bull. 33, 4618-4620
- Ikehara, M., Fujimoto, K., Aoyama, Y., Yanase, K., Matsugi, J., Inaoka, T., Tokunaga, T., Uesugi, S., Iwai, S., and Ohtsuka, E. (1986) Synthesis of a gene coding for human lysozyme. Chem. Pharm. Bull. 34, 2202-2208
- Itoh, H., Kozasa, T., Nagata, S., Nakamura, S., Katada, T., Ui, M., Iwai, S., Ohtsuka, E., Kawasaki, H., Suzuki, K., and Kaziro, Y. (1986) Molecular cloning and sequence determination of cDNAs for alfa subunits of the guanine nucleotide-binding proteins Gs, Gi, and Go from rat brain. Proc. Natl. Acad. Sci. USA 83, 3776-3780
- Chisaka, O., Iwai, S., Ohtsuka, E., and Matsubara, K. (1986) Cloning a defined region of DNA using a limited action of DNA polymerase: Application to dissection of hepatitis B virus surface antigen gene. Gene 45, 19-25
- Kawase, Y., Iwai, S., Inoue, H., Miura, K., and Ohtsuka, E. (1986) Studies on nucleic acid interactions. I. Stabilities of mini-duplexes (dG2A4XA4G2 ・ dC2T4YT4C2) and self-complementary d(GGGAAXYTTCCC) containing deoxyinosine and other mismatched bases. Nucleic Acids Res. 14, 7727-7736
- Iwai, S., Imura, A., Inoue, Y., Inoue, H., Miura, K., Tokunaga, T., Ikehara, M., and Ohtsuka, E. (1986) Deoxyribonucleic acids and related compounds. XXII. Synthesis of genes for human nerve growth factor and its fused protein. Chem. Pharm. Bull. 34, 4724-4730
- Miura, K., Inoue, Y., Nakamori, H., Iwai, S., Ohtsuka, E., Ikehara, M., Noguchi, S., and Nishimura, S. (1986) Synthesis and expression of a synthetic gene for the activated human c-Ha-ras protein. Jpn. J. Cancer Res. 77, 45-51
- Iwai, S., Koizumi, M., Ikehara, M., and Ohtsuka, E. (1987) Solid-phase synthesis of protected oligonucleotide blocks: Applications to block condensation on a polymer support and synthesis of a 3′-modified oligonucleotide. Tetrahedron 43, 59-67
- Kuchino, Y., Mori, F., Kasai, H., Inoue, H., Iwai, S., Miura, K., Ohtsuka, E., and Nishimura, S. (1987) Misreading of DNA templates containing 8-hydroxydeoxyguanosine at the modified base and at adjacent residues. Nature 327, 77-79
- Iwai, S., Yamada, E., Asaka, M., Hayase, Y., Inoue, H., and Ohtsuka, E. (1987) A new solid-phase synthesis of oligoribonucleotides by the phosphoro-p-anisidate method using tetrahydrofuranyl protection of 2′-hydroxyl groups. Nucleic Acids Res. 15, 3761-3772
- Inoue, H., Hayase, Y., Iwai, S., and Ohtsuka, E. (1987) Sequence-dependent hydrolysis of RNA using modified oligonucleotide splints and RNase H. FEBS Lett. 215, 327-330
- Inoue, H., Hayase, Y., Imura, A., Iwai, S., Miura, K., and Ohtsuka,E. (1987) Synthesis and hybridization studies on two complementary nona(2′-O-methyl)ribonucleotides. Nucleic Acids Res. 15, 6131-6148
- Kawase, Y., Iwai, S., and Ohtsuka, E. (1988) Synthesis and properties of hexanucleotides containing deoxyinosine. Chem. Pharm. Bull. 36, 118-121
- Koizumi, M., Iwai, S., and Ohtsuka, E. (1988) Construction of a series of several self-cleaving RNA duplexes using synthetic 21-mers. FEBS Lett. 228, 228-230
- Iwai, S. and Ohtsuka, E. (1988) Synthesis of oligoribonucleotides by the phosphoramidite approach using 5′-levulinyl and 2′-tetrahydrofuranyl protection. Tetrahedron Lett. 29, 5383-5386
- Iwai, S. and Ohtsuka, E. (1988) 5′-Levulinyl and 2′-tetrahydrofuranyl protection for the synthesis of oligoribonucleotides by the phosphoramidite approach. Nucleic Acids Res. 16, 9443-9456
- Koizumi, M., Iwai, S., and Ohtsuka, E. (1988) Cleavage of specific sites of RNA by designed ribozymes. FEBS Lett. 239, 285-288
- Kawase, Y., Iwai, S., and Ohtsuka, E. (1989) Synthesis and thermal stability of dodecadeoxyribonucleotides containing deoxyinosine pairing with four major bases. Chem. Pharm. Bull. 37, 599-601
- Kawase, Y., Koizumi, M., Iwai, S., and Ohtsuka, E. (1989) Synthesis of wobble pairing oligoribonucleotides. Chem. Pharm. Bull. 37, 2313-2317
- Koizumi, M., Hayase, Y., Iwai, S., Kamiya, H., Inoue, H., and Ohtsuka, E. (1989) Design of RNA enzymes distinguishing a single base mutation in RNA. Nucleic Acids Res. 17, 7059-7071
- Kanaya, S., Kohara, A., Miura, Y., Sekiguchi, A., Iwai, S., Inoue, H., Ohtsuka, E., and Ikehara, M. (1990) Identification of the amino acid residues involved in an active site of Escherichia coli ribonuclease H by site-directed mutagenesis. J. Biol. Chem. 265, 4615-4621
- Katahira, M., Lee, S. J., Kobayashi, Y., Sugeta, H., Kyogoku, Y., Iwai, S., Ohtsuka, E., Benevides, J. M., and Thomas, G. J., Jr. (1990) Structure in solution of the RNA・DNA hybrid (rA)8・(dT)8 determined by NMR and Raman spectroscopy. J. Am. Chem. Soc. 112, 4508-4512
- Iwai, S., Sasaki, T., and Ohtsuka, E. (1990) Large scale synthesis of oligoribonucleotides on a solid support: Synthesis of a catalytic RNA duplex. Tetrahedron 46, 6673-6688
- Murata, T., Iwai, S., and Ohtsuka, E. (1990) Synthesis and characterization of a substrate for T4 endonuclease V containing a phosphorodithioate linkage at the thymine dimer site. Nucleic Acids Res. 18, 7279-7286
- Nakamura, H., Oda, Y., Iwai, S., Inoue, H., Ohtsuka, E., Kanaya, S., Kimura, S., Katsuda, C., Katayanagi, K., Morikawa, K., Miyashiro, H., and Ikehara, M. (1991) How does RNase H recognize a DNA・RNA hybrid ? Proc. Natl. Acad. Sci. USA 88, 11535-11539
- Murata, T., Iwai, S., and Ohtsuka, E. (1992) Synthesis of a dodecadeoxyribooligonucleotide containing a 3′-thio analogue of thymidine photodimer. Heterocycles 33, 529-531
- Hori, N., Iwai, S., Inoue, H., and Ohtsuka, E. (1992) Photoaffinity labeling of T4 endonuclease V with a substrate containing a phenyldiazirine derivative. J. Biol. Chem. 267, 15591-15594
- Iwai, S., Pritchard, C., Mann, D. A., Karn, J., and Gait, M. J. (1992) Recognition of the high affinity binding site in rev-response element RNA by the human immunodeficiency virus type-1 rev protein. Nucleic Acids Res. 20, 6465-6472
- Hamy, F., Asseline, U., Grasby, J., Iwai, S., Pritchard, C., Slim, G., Butler, P. J. G., Karn, J., and Gait, M. J. (1993) Hydrogen-bonding contacts in the major groove are required for human immunodeficiency virus type-1 tat protein recognition of TAR RNA. J. Mol. Biol. 230, 111-123
- Kamiya, H., Murata, N., Murata, T., Iwai, S., Matsukage, A., Masutani, C., Hanaoka, F., and Ohtsuka, E. (1993) Cyclobutane thymine dimers in a ras proto-oncogene hot spot activate the gene by point mutation. Nucleic Acids Res. 21, 2355-2361
- Oda, Y., Iwai, S., Ohtsuka, E., Ishikawa, M., Ikehara, M., and Nakamura, H. (1993) Binding of nucleic acids to E. coli RNase HI observed by NMR and CD spectroscopy. Nucleic Acids Res. 21, 4690-4695
- Lee, B. J., Sakashita, H., Ohkubo, T., Ikehara, M., Doi, T., Morikawa, K., Kyogoku, Y., Osafune, T., Iwai, S., and Ohtsuka, E. (1994) Nuclear magnetic resonance study of the interaction of T4 endonuclease V with DNA. Biochemistry 33, 57-64
- Iwai, S., Maeda, M., Shimada, Y., Hori, N., Murata, T., Morioka, H., and Ohtsuka, E. (1994) Endonuclease V from bacteriophage T4 interacts with its substrate in the minor groove. Biochemistry 33, 5581-5588
- Uchiyama, Y., Miura, Y., Inoue, H., Ohtsuka, E., Ueno, Y., Ikehara, M., and Iwai, S. (1994) Studies of the interactions between Escherichia coli ribonuclease HI and its substrate. J. Mol. Biol. 243, 782-791
- Uchiyama, Y., Iwai, S., Ueno, Y., Ikehara, M., and Ohtsuka, E. (1994) Role of the Mg2+ ion in the Escherichia coli ribonuclease HI reaction. J. Biochem. 116, 1322-1329
- Iwai, S., Maeda, M., Shirai, M., Shimada, Y., Osafune, T., Murata, T., and Ohtsuka, E. (1995) Reaction mechanism of T4 endonuclease V determined by analysis using modified oligonucleotide duplexes. Biochemistry 34, 4601-4609
- Iwai, S., Kataoka, S., Wakasa, M., Ohtsuka, E., and Nakamura, H. (1995) Recognition of 2′-hydroxyl groups by Escherichia coli ribonuclease HI. FEBS Lett. 368, 315-320
- Vassylyev, D. G., Kashiwagi, T., Mikami, Y., Ariyoshi, M., Iwai, S., Ohtsuka, E., and Morikawa, K. (1995) Atomic model of a pyrimidine dimer excision repair enzyme complexed with a DNA substrate: Structural basis for damaged DNA recognition. Cell 83, 773-782
- Nishizaki, T., Iwai, S., Ohkubo, T., Kojima, C., Nakamura, H., Kyogoku, Y., and Ohtsuka, E. (1996) Solution structures of DNA duplexes containing a DNA・RNA hybrid region, d(GG)r(AGAU)d(GAC) ・ d(GTCATCTCC) and d(GGAGA)r(UGAC) ・ d(GTCATCTCC). Biochemistry 35, 4016-4025
- Iwai, S., Shimizu, M., Kamiya, H., and Ohtsuka, E. (1996) Synthesis of a phosphoramidite coupling unit of the pyrimidine (6-4) pyrimidone photoproduct and its incorporation into oligodeoxynucleotides. J. Am. Chem. Soc. 118, 7642-7643
- Iwai, S., Wakasa, M., Ohtsuka, E., Kanaya, S., Kidera, A., and Nakamura, H. (1996) Interaction of the basic protrusion of Escherichia coli ribonuclease HI with its substrate. J. Mol. Biol. 263, 699-706
- Nakaya, T., Iwai, S., Fujinaga, K., Sato, Y., Ohtsuka, E., and Ikuta, K. (1997) Decoy approach using RNA-DNA chimera oligonucleotides to inhibit the regulatory function of human immunodeficiency virus type 1 Rev protein. Antimicrob. Agents Chemother. 41, 319-325
- Fujiwara, Y. and Iwai, S. (1997) Thermodynamic studies of the hybridization properties of photolesions in DNA. Biochemistry 36, 1544-1550
- Nishizaki, T., Iwai, S., Ohtsuka, E., and Nakamura, H. (1997) Solution structure of an RNA・2′-O-methylated RNA hybrid duplex containing an RNA・DNA hybrid segment at the center. Biochemistry 36, 2577-2585
- Hitomi, K., Kim, S.-T., Iwai, S., Harima, N., Otoshi, E., Ikenaga, M., and Todo, T. (1997) Binding and catalytic properties of Xenopus (6-4) photolyase. J. Biol. Chem. 272, 32591-32598
- Nakajima, S., Sugiyama, M., Iwai, S., Hitomi, K., Otoshi, E., Kim, S.-T., Jiang, C.-Z., Todo, T., Britt, A. B., and Yamamoto, K. (1998) Cloning and characterization of a gene (UVR3) required for photorepair of 6-4 photoproducts in Arabidopsis thaliana. Nucleic Acids Res. 26, 638-644
- Kamiya, H., Iwai, S., and Kasai, H. (1998) The (6-4) photoproduct of thymine-thymine induces targeted substitution mutations in mammalian cells. Nucleic Acids Res. 26, 2611-2617
- Sugasawa, K., Ng, J. M. Y., Masutani, C., Iwai, S., van der Spek, P. J., Eker, A. P. M., Hanaoka, F., Bootsma, D., and Hoeijmakers, J. H. J. (1998) Xeroderma pigmentosum group C protein complex is the initiator of global genome nucleotide excision repair. Mol. Cell 2, 223-232
- Mizukoshi, T., Hitomi, K., Todo, T., and Iwai, S. (1998) Studies on the chemical synthesis of oligonucleotides containing the (6-4) photoproduct of thymine-cytosine and its repair by (6-4) photolyase. J. Am. Chem. Soc. 120, 10634-10642
- Iwai, S., Mizukoshi, T., Fujiwara, Y., Masutani, C., Hanaoka, F., and Hayakawa, Y. (1999) Benzimidazolium triflate-activated synthesis of (6-4) photoproduct-containing oligonucleotides and its application. Nucleic Acids Res. 27, 2299-2303
- Masutani, C., Araki, M., Yamada, A., Kusumoto, R., Nogimori, T., Maekawa, T., Iwai, S., and Hanaoka, F. (1999) Xeroderma pigmentosum variant (XP-V) correcting protein from HeLa cells has a thymine dimer bypass DNA polymerase activity. EMBO J. 18, 3491-3501
- Masutani, C., Kusumoto, R., Yamada, A., Dohmae, N., Yokoi, M., Yuasa, M., Araki, M., Iwai, S., Takio, K., and Hanaoka, F. (1999) The XPV (xeroderma pigmentosum variant) gene encodes human DNA polymerase eta. Nature 399, 700-704
- Fujiwara, Y., Masutani, C., Mizukoshi, T., Kondo, J., Hanaoka, F., and Iwai, S. (1999) Characterization of DNA recognition by the human UV-damaged DNA-binding protein. J. Biol. Chem. 274, 20027-20033
- Kanno, S., Iwai, S., Takao, M., and Yasui, A. (1999) Repair of apurinic/apyrimidinic sites by UV damage endonuclease; a repair protein for UV and oxidative damage. Nucleic Acids Res. 27, 3096-3103
- Ohtsubo, T., Nishioka, K., Imaiso, Y., Iwai, S., Shimokawa, H., Oda, H., Fujiwara, T., and Nakabeppu, Y. (2000) Identification of human MutY homolog (hMYH) as a repair enzyme for 2-hydroxyadenine in DNA and detection of multiple forms of hMYH located in nuclei and mitochondria. Nucleic Acids Res. 28, 1355-1364
- Hitomi, K., Okamoto, K., Daiyasu, H., Miyashita, H., Iwai, S., Toh, H., Ishiura, M., and Todo, T. (2000) Bacterial cryptochrome and photolyase: Characterization of two photolyase-like genes of Synechocystis sp. PCC6803. Nucleic Acids Res. 28, 2353-2362
- Masutani, C., Kusumoto, R., Iwai, S., and Hanaoka, F. (2000) Mechanisms of accurate translesion synthesis by human DNA polymerase eta. EMBO J. 19, 3100-3109
- Yamada, A., Masutani, C., Iwai, S., and Hanaoka, F. (2000) Complementation of defective translesion synthesis and UV light sensitivity in xeroderma pigmentosum variant cells by human and mouse DNA polymerase eta. Nucleic Acids Res. 28, 2473-2480
- Ohashi, E., Ogi, T., Kusumoto, R., Iwai, S., Masutani, C., Hanaoka, F., and Ohmori, H. (2000) Error-prone bypass of certain DNA lesions by the human DNA polymerase kappa. Genes Dev. 14, 1589-1594
- Kobayashi, Y., Ishikawa, T., Hirayama, J., Daiyasu, H., Kanai, S., Toh, H., Fukuda, I., Tsujimura, T., Terada, N., Kamei, Y., Yuba, S., Iwai, S., and Todo, T. (2000) Molecular analysis of zebrafish photolyase/cryptochrome family: Two types of cryptochromes present in zebrafish. Genes Cells 5, 725-738
- Tissier, A., Frank, E. G., McDonald, J. P., Iwai, S., Hanaoka, F., and Woodgate, R. (2000) Misinsertion and bypass of thymine-thymine dimers by human DNA polymerase iota. EMBO J. 19, 5259-5266
- Iwai, S. (2000) Synthesis of thymine glycol containing oligonucleotides from a building block with the oxidized base. Angew. Chem. Int. Ed. 39, 3874-3876
- Haruki, M., Tsunaka, Y., Morikawa, M., Iwai, S., and Kanaya, S. (2000) Catalysis by Escherichia coli ribonuclease HI is facilitated by a phosphate group of the substrate. Biochemistry 39, 13939-13944
- Kusumoto, R., Masutani, C., Sugasawa, K., Iwai, S., Araki, M., Uchida, A., Mizukoshi, T., and Hanaoka, F. (2001) Diversity of the damage recognition step in the global genomic nucleotide excision repair in vitro. Mutation Res. 485, 219-227
- Sugasawa, K., Okamoto, T., Shimizu, Y., Masutani, C., Iwai, S., and Hanaoka, F. (2001) A multistep damage recognition mechanism for global genomic nucleotide excision repair. Genes Dev. 15, 507-521
- Hitomi, K., Nakamura, H., Kim, S.-T., Mizukoshi, T., Ishikawa, T., Iwai, S., and Todo, T. (2001) Role of two histidines in the (6-4) photolyase reaction. J. Biol. Chem. 276, 10103-10109
- Ura, K., Araki, M., Saeki, H., Masutani, C., Ito, T., Iwai, S., Mizukoshi, T., Kaneda, Y., and Hanaoka, F. (2001) ATP-dependent chromatin remodeling facilitates nucleotide excision repair of UV-induced DNA lesions in synthetic dinucleosomes. EMBO J. 20, 2004-2014
- Ishikawa, T., Uematsu, N., Mizukoshi, T., Iwai, S., Iwasaki, H., Masutani, C., Hanaoka, F., Ueda, R., Ohmori, H., and Todo, T. (2001) Mutagenic and nonmutagenic bypass of DNA lesions by Drosophila DNA polymerases dpol eta and dpol iota. J. Biol. Chem. 276, 15155-15163
- Iwai, S. (2001) Synthesis and thermodynamic studies of oligonucleotides containing the two isomers of thymine glycol. Chem. Eur. J. 7, 4343-4351
- Boudsocq, F., Iwai, S., Hanaoka, F., and Woodgate, R. (2001) Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4): an archaeal DinB-like DNA polymerase with lesion-bypass properties akin to eukaryotic pol eta. Nucleic Acids Res. 29, 4607-4616
- Mizukoshi, T., Kodama, T. S., Fujiwara, Y., Furuno, T., Nakanishi, M., and Iwai, S. (2001) Structural study of DNA duplexes containing the (6-4) photoproduct by fluorescence resonance energy transfer. Nucleic Acids Res. 29, 4948-4954
- Sugasawa, K., Shimizu, Y., Iwai, S., and Hanaoka, F. (2002) A molecular mechanism for DNA damage recognition by the xeroderma pigmentosum group C protein complex. DNA Repair 1, 95-107
- Sakai, Y., Furuichi, M., Takahashi, M., Mishima, M., Iwai, S., Shirakawa, M., and Nakabeppu, Y. (2002) A molecular basis for the selective recognition of 2-hydroxy-dATP and 8-oxo-dGTP by human MTH1. J. Biol. Chem. 277, 8579-8587
- Kusumoto, R., Masutani, C., Iwai, S., and Hanaoka, F. (2002) Translesion synthesis by human DNA polymerase eta across thymine glycol lesions. Biochemistry 41, 6090-6099
- Hey, T., Lipps, G., Sugasawa, K., Iwai, S., Hanaoka, F., and Krauss, G. (2002) The XPC-HR23B complex displays high affinity and specificity for damaged DNA in a true-equilibrium fluorescence assay. Biochemistry 41, 6583-6587
- Otsuka, C., Kobayashi, K., Kawaguchi, N., Kunitomi, N., Moriyama, K., Hata, Y., Iwai, S., Loakes, D., Noskov, V.N., Pavlov, Y., and Negishi, K. (2002) Use of yeast transformation by oligonucleotides to study DNA lesion bypass in vivo. Mutation Res. 502, 53-60
- Takahashi, M., Maraboeuf, F., Sakai, Y., Yakushiji, H., Mishima, M., Shirakawa, M., Iwai, S., Hayakawa, H., Sekiguchi, M., and Nakabeppu, Y. (2002) Role of tryptophan residues in the recognition of mutagenic oxidized nucleotides by human antimutator MTH1 protein. J. Mol. Biol. 319, 129-139
- Servant, L., Cazaux, C., Bieth, A., Iwai, S., Hanaoka, F., and Hoffmann, J.-S. (2002) A role for DNA polymerase beta in mutagenic UV lesion bypass. J. Biol. Chem. 277, 50046-50053
- Shimizu, Y., Iwai, S., Hanaoka, F., and Sugasawa, K. (2003) Xeroderma pigmentosum group C protein interacts physically and functionally with thymine DNA glycosylase. EMBO J. 22, 164-173
- Futami, T., Miyagishi, M., Iwai, S., Seki, M., and Taira, K. (2003) Stimulatory effect of an indirectly attached RNA helicase-recruiting sequence on the suppression of gene expression by antisense oligonucleotides. Antisense Nucleic Acid Drug Dev. 13, 9-17